Question

In confocal scanning laser microscope, the image is built up pixel by pixel by collecting the emitted Photon from which compound that acts like a dye present in the sample? ​

Answer 1

Answer:

The CLSM works by passing a laser beam through a light source aperture which is then focused by an objective lens into a small area on the surface of your sample and an image is built up pixel-by-pixel by collecting the emitted photons from the fluorophores in the sample.