in genetic engineering, after having cloned the gene of interest and having optimised the condition to induce the expression of the target protein, one has to consider producing it on a large scale. can you think of any reason why there is a need for large scale production?
if you explained correctly I will mark you as brainliest...! please don't send Silly answers
Answers
Answer:
Cloning allows would‐be parents to give their children the same genes as a pre‐existing person, whereas genetic engineering allows them to give their children genes that have been intentionally designed, modified or selected in the laboratory for some particular purpose.
We should not use bacterial cells to clone the eukaryotic genes because they do not have splicing machinery and post translational modification system. ... Bacterial cells have the limited genome size and due to this they do not
Answer:
Explanation:
One way of detecting a specific cloned gene is by detecting its protein product expressed in the bacterial cell. Therefore, in these cases, it is necessary to be able to express the gene in bacteria; that is, to transcribe it and translate the mRNA into protein. In standard molecular cloning experiments, the cloning of any DNA fragment essentially involves seven steps: Choice of host organism and cloning vector, (2) Preparation of vector DNA, (3) Preparation of DNA to be cloned, (4) Creatio of recombinant DNA, (5) Introduction of recombinant DNA into host organism