in one mm stage micrometer,9.9 stage micrometer equals 10 ocular micrometer division .calculate the calibration factor. when the pollen grains occupies 5 ocular division ,what would be its length?
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Measurement of the dimensions of microorganisms is done under microscope with the help of two micro-scales called ‘micrometers’. Both the micrometers have microscopic graduations etched on their surfaces. One of them, the ‘ocular micrometer’ is a circular glass disc, which fits into the circular shelf inside the eyepiece.
It has arbitrary graduations etched on its surface. However, the distance between the etched graduations is constant for a particular ocular micrometer. The other micrometer, called ‘stage micrometer’, is a special glass slide, which is clipped to the stage of the microscope. It has standard graduations etched on its surface, which are 10 µ apart.
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