In pbr322, foreign dna has to be introduced in tetrregion. From the restriction enzymes given below, which one should be used and why: pvui, ecori, bamhi (b) give reasons, why the other two enzymes cannot be used. Ansers
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(a)The restriction enzymes that should be used are Bam HI , as restriction site for this enzyme is present in tetR region.
Bam HI is a type II restriction endonuclease, having the capacity for recognizing short sequences of DNA and specifically cleaving them at a target site.
(b) PvuI will not be used, as restriction site for this enzyme is present in ampR region (not in tetR)
EcoRI is a restriction endonuclease enzyme isolated from species E. coli and also not used.The Eco part of the enzyme's name originates from the species from which it was isolated,
Bam HI is a type II restriction endonuclease, having the capacity for recognizing short sequences of DNA and specifically cleaving them at a target site.
(b) PvuI will not be used, as restriction site for this enzyme is present in ampR region (not in tetR)
EcoRI is a restriction endonuclease enzyme isolated from species E. coli and also not used.The Eco part of the enzyme's name originates from the species from which it was isolated,
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