Question

In protein test of acetic acid white precipitate appears .give reason

Answer 1

Take 5 m L of concentrated n-i-t-r-i-c acid in a test tube.

• Incline the tube and add the urine sample with a dropper, so

that the latter flows down slowly along the side of the test tube

to form a separate layer.

• A white ring develops at the junction of the two liquids which

indicates the presence of albumin in the urine sample.

Principle: N-i-t-r-i-c acid causes the precipitation of albumin. When heated or treated with s-u-l-p-h-o-s-a-l-i-c-y-l-i-c acid, albumin undergoes coagulation. ... A white ring develops at the junction of the two liquids which indicates the presence of albumin in the urine sample.

Answer 2

Presence of phosphates, and carbonates are responsible for formation of precipitate during acetic acid test.

Explanation:

• Acetic acid test is done to detect the albumin protein in urine. The urine is heated until it becomes cloudy and several drops of acetic acid are added.
• The proteins are denatured and coagulated on heating which gives a white cloudy precipitate in the reaction.
• The presence of carbonates, and phosphates are responsible for giving a white precipitate or cloudy appearance in the test.

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