IN RDNA TECHNOLOGY WHAT IS THE ROLE OF DNA LIGASES
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Ligases catalyze the joining of nucleic acid molecules enzymatically in an ATP-dependent manner. T4 DNA and RNA ligases are members of a large family of ligases and are derived from the T4 bacteriophage representing the most commonly used ligases in cloning protocols.
Thermo Scientific ligase selection table
T4 DNA LigaseT4 RNA LigaseApplicationsJoining dsDNA with cohesive or blunt terminiJoining of ds oligonucleotide linkers or adaptors to dsDNARepairing nicks in duplex DNA, RNA or DNA-RNA hybridsLigase-mediated RNA detectionSite-directed mutagenesisAmplify fragment length polymorphismsRNA 3'-end labeling with cytidine 3',5'-bis [alpha-32P] phosphateJoining RNA to RNACircularization of synthetic oligonucleotidesSpecific modifications of tRNAsOligodeoxyribonucleotide ligation to single-stranded cDNAs for 5'-RACESite-specific generation of composite primers for PCR
Thermo Scientific ligase selection table
T4 DNA LigaseT4 RNA LigaseApplicationsJoining dsDNA with cohesive or blunt terminiJoining of ds oligonucleotide linkers or adaptors to dsDNARepairing nicks in duplex DNA, RNA or DNA-RNA hybridsLigase-mediated RNA detectionSite-directed mutagenesisAmplify fragment length polymorphismsRNA 3'-end labeling with cytidine 3',5'-bis [alpha-32P] phosphateJoining RNA to RNACircularization of synthetic oligonucleotidesSpecific modifications of tRNAsOligodeoxyribonucleotide ligation to single-stranded cDNAs for 5'-RACESite-specific generation of composite primers for PCR
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