Question

in regards to target DNA used in inverted Pcr whichof the following is not true statement?
A. intact known segments
B. resyticted segments
C. blunt ended segments
D. flanked unknown segments flanked on either side​

Answer 1

Answer:

option b.

Explanation:

The target DNA is cut with a restriction enzyme that produces sticky ends and that does not cut within the region of which the sequence is known. The cutter instead cuts at unknown sites on the either sides of the known sequence. This lets the DNA circularize later, which is a very important step for achieving our desired results.