In Sahli's method of haemoglobin estimation , can you use H2SO4 or HNO3 ( not in concentrate form) instead of HCL?
Answers
Cyanmethemoglobin method (calorimetric)
Oxyhemoglobin method
Alkali hematin method
Halden carboxy haemoglobin method
Gasometric method
Specific gravity method
Tallquist method
Auto analyzers
3. Which is the best method for Hb estimation
Cyanmethemoglobin method
4. Which anticoagulant should be used for Hb estimation
Ethylenediamine tetra acetic acid(EDTA)
5. How much quantity of EDTA Should be Used for 1 ml of blood
1.0- 1.2 mg/ml of blood
6. How N/10 HCL is prepared
Concentrated HCl-4.5 ml
Distilled Water-500ml
To make 500 ml of N/10 HCL
7. What difference would it make if N/10 HCL is taken above or below the 20% mark?
If N/10 HCL more – The colour of undiluted solution may be lighter than standard.
If N/10 HCL less –All haemoglobin will not get converted.
8. What is the principle of sahlis method
Hemoglobin is converted by HCl into acid haematin which gives brown colur to solution
This colour of the solution is matched with the colour of the comparator of sahlis haemoglobinometer
9. What is the procedure of Acid hematin method
N/10 HCl is taken up to 20 mark of the gratuated tube
About 20 cc of blood is taken in the hemoglobin pipette
The tip of pipette is wiped with blotting paper
The blood is added to the HCl in the Hemoglobinometre tube
Wait for 10 minutes and then add distilled water drop by drop till the colour matches with the colour of comparator of sahlis haemoglobinometer
Haemoglobin is expressed in gm%
10. In sahlis method ,why do we wait for 10 minutes after mixing blood with acid
RBCs are lysed and Hb gets converted in to acid haematin completely
11. In sahlis Hb meter apparatus, why are square tube and flat comparator glasses preferred?
It is easier to compare adjoining flat surfaces ex: Square tube with acid haematin with flat brown surface on either side. Error due to curvature is avoided
12. Which precaution should be taken during the procedure
Wait for atleast 10 min for formation of Acid hematin
Tap water cannot be used as the pH varies and accurate values may not be obtained
Remove stirrer while adding distilled water and also while taking reading
Hemoglobinometer should be hold at the eye level against good light and lower meniscus of the Hemoglobinometer tube solution should be considered.
13. Why N/10 HCL used in Sahlis method
Brown colour of the comparator glass is equivalent to the colour of acid haematin produced using N/10 Hcl for blood sample of 14.8 gm%. Test solution is compared against it. Therefore no other strength of HCL Should be used
14. What are the advantages of Sahlis method
Simple bed side test
Reagents and apparatus are cheap
Easy to perform
Quick and inexpensive
15. What are the disadvantages of Sahlis method
Time factor
The color of acid hematin fades quickly with passage of time
Color of the standard comparator fades with passage of time
Acid hematin solution is not stable
Color Matching: Variation from person to person in matching the color
Technical errors:
Improper mixing of blood
Tissue fluid containing capillary blood
Errors in pipette calibration ,sample and equipment,
Cannot estimate all different types of haemoglobin(carboxy, meth,and supha)
This method is not suitable for fetal haemoglobin which is not converted in to acid hematin
Non hemoglobin substances like protein and lipids in the plasma influence the colour of the blood that is diluted with acid
16. How much blood is used in cyanmet Hb estimation method
20 microliter
17. Which reagent is used in cyanmet Hb method?
Drabkin solution
18. Drabkin solution composition?
Drabkin’s solution PH-7.0-7.4
Potassium cyanide – 50mg
Potassium ferricyanide – 200mg
Potassium dihydrogen phosphate – 200mg
Distilled water 1L,
Non ionic detergent 1ml.
The final solution should be clear and pale yellow in colour.
19. What is the principle of cyan met Hb method
First Hb is converted to methemoglobin by potassium ferricyanide and then KCN converts it to cyanm
No.
Explanation:
- Sahli's method of haemoglobin estimation is used medically to determine the pathogen infection in the blood of an individual.
- The main principle of this method is the conversion of hemoglobin into hematin on addition of hydrochloric acid. The hematin is brown color compound.
- The nitric acid or sulfuric acid cannot convert hemoglobin into hematin and no brown color compound is formed by using these reagents.
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