In Sanger Sequencing method what happened if we use only one strand as template and what happened if we use two of the strands as template?
Answers
Answered by
1
Answer:
Because only one primer is used, only one strand is copied during sequencing, there is a linear increase of the number of copies of one strand of the gene. Therefore, there has to be a large amount of copies of the gene in the starting mixture for sequencing.
Similar questions