In the activities related to photosynthesis, the leaf is boiled in water and then in alcohol. answer the following questions
[i] Why does the colour of alcohol turn green though the colour of water does not change?
[ii] How is the manner of heating of water-container and alcohol-container different? Why?
[iii] How does iodine test help us to reach conclusions?
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- The hot water kills the leaf and the alcohol breaks down the chlorophyll, taking the green color out of the leaf
- Alcohol is highly inflammable and its vapors are explosive, therefore, limiting heat applies becomes necessary. The bath with the boiling water produces a constant temperature of just a few degrees over the boiling point of the isopropyl alcohol, so to restrict boiling of the alcohol to a sustained rate.
- Iodine is an indicator that turns blue-black in the presence of starch. The leaf that was in the light turns blue-black, which demonstrates that the leaf has been performing photosynthesis and producing starch. Try the test again with a variegated leaf (one with both green and white) that has been in the sunlight.
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