Question

In the experiment of wearing a temporary mount of a leaf peel to observe stomata we use glycerine and suffering why

Answer 1

Role in the preparation of temporary mount of a leaf peel to show stomata:

Glycerine:

It is a good dehydrating agent and prevents the specimen from drying out.

Due to its refractive nature, it tends to reflect the light better and as a consequence, the specimen can be clearly observed under the microscope.

Safranin:

It stains the lignin and suberin and other plant materials easily.

It imparts a red colour to the cells and tissues, thereby highlighting them when seen under the microscope.