Question

In the genome sequence of Propionibacterium acnes (bacterium that causes acne), you have discovered a gene predicted to encode a lipase. This is an enzyme that breaks down lipids in the skin, and if an inhibitor could be developed, it might lead to a therapy for acne. You want to find out more about this enzyme. You plan to

1. clone the gene

2. express it in E. coli

3. make lots of the protein and study its properties

How would you clone this gene into E. coli?

            -- assume you have a suitable cloning vector (pCR2.1 from Invitrogen; see next page)

            -- assume you can use the techniques you learned about so far

The key here is how to figure out how you would get that gene, and only that gene.

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Answer 1

Explanation:

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Answer 2

Explanation:

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