In the hybridization step of dna fingerprinting technique, the dna bands are flooded with
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Answer:
he glass tubes are placed horizontally in the oven on a wheel which moves slowly around. In the early days of DNA fingerprinting, instead of a hybridisation oven, Tupperware containers were used for 65 degree Celsius stage, and the paper was washed in plastic seed trays.
The film cassette is taken into the dark room and opened. The film can be either held with a gloved hand or placed in a metal frame. It is then dunked in three chambers “developer”, “stop” and “fix”, in the same way as traditional photograph developing. The bands appear slowly in the developer – you take it out occasionally and check it by holding it up against the red light – if the intensity of the bands is good then you dunk in “stop” then “fix”.
The developed film is then taken to the lab and examined on a white light box (horizontally placed on a lab bench, not vertically, not like “House”). The name and date, and details of the samples would be written in pen.
The key to the DNA fingerprint is the probe, the radioactive bit of DNA that identifies lots of fragments that contain the “minisatellite repeats”. These repeats have the 33 letters of DNA that are used in the probe but repeated lots of times. The number of repeats differ between different people. So the DNA fragment sizes with these different sized-repeats are different for different people – hence different black bands on a film for different people.
Explanation:
⚫It provides evidence acceptable in court.
⚫Paternity test-It help to decide true father or mother of child.
⚫It helps in tumor analysis.
⚫It helps to solve problems in immigration