In thin layer chromatography sample, A, B, C and D travelled 0.5 cm, 1.5 cm, 2.0 cm and 2.5 cm, respectively when mobile phase solvent travelled 4.0 cm. Calculate the Rf value for A, B, C and D samples.
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Answer:
the chromatography is the technique tonseperate colour and this are dependent on its darkness.
Explanation:
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Answer:
Thin layer chromatography (TLC) is a chromatographic technique used to separate the components of a mixture using a thin stationary phase supported by an inert backing. It may be performed on the analytical scale as a means of monitoring the progress of a reaction, or on the preparative scale to purify small amounts of a compound. TLC is an analytical tool widely used because of its simplicity, relative low cost, high sensitivity, and speed of separation.TLC functions on the same principle as all chromatography: a compound will have different affinities for the mobile and stationary phases, and this affects the speed at which it migrates. The goal of TLC is to obtain well defined, well separated spots.
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