Question

In tissue culture which one of the following pairs of substances are used to induce shoot and root formation respectively in the callus ______ a) Cytokinin and auxin
b) auxin and cytokinin
c) PEG and IAA
d) ethylene and ABA

Answer 1

auxin and cytokinin are used for shoot and root formation

Answer 2

Hii Manasi,

● Answer -
In tissue culture, *cytokinin and auxin* substances are used to induce shoot and root formation respectively in the callus.

◆ Extra information -
- Callus is a mass of undifferentiated cells that arise from plant cell/tissue in artificial culture.
- Skoog & Miller discovered that auxins & cytokinins induce root & shoot formation respectively.
- Cell differentiation in plant tissue culture is controlled by ratio of cytokinins/auxins.


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