Question

in vitro amplification of dNA segment is called​

Answer 1

Answer:

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Explanation:

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Answer 2

Answer:

It is called Polymerase Chain Reaction (PCR).

Explanation:

• PCR is developed by the scientist, Kary Mullis in 1983. It is a laboratory technique used for the amplification of DNA fragments for different research purposes.
• It is divided into three steps; denaturation, annealing, and elongation.
• Denaturation - This step breaks the hydrogen bonds between the strand and converts ds DNA to ss DNA. It occurs at 94-95℃ for 30 seconds.
• Annealing - In this step, primers bind to their complementary sequences on the template DNA. The temperature is 56-63℃ for 30 seconds.
• Elongation - At this step, bases are added to the 3’ end of the primer by the Taq polymerase (extracted from Thermus aquaticus). The temperature rises to 72-75℃ for 1 minute.
• All three steps are repeated for 20-40 cycles to obtain much DNA of interest in a very short time.
• PCR has applications in rDNA technology. It is used for cloning, DNA fingerprinting, forensic investigations, DNA or RNA detection of pathogenic organisms, etc.

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