Question

In which method, a probe is allowed to hybridise to its
complementary DNA in the clone of cells?
(A) Gene therapy
(B) Recombinant DNA technology
(C) Polymerase chain reaction
(D) Enzyme Linked Immuno-Sorbent Assay
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Answer 1

I think the answer is B

Explanation:

A single stranded DNA or RNA joined with a radioactive molecule (probe) is allowed to hybridise to its complementary DNA in a clone of cells. It is followed by detection using autoradiography. The clone having the mutated gene will not appear on the photographic film, because the probe will not have the complementarity with the mutated gene.

Answer 2

Option B is the correct answer.

• In recombinant DNA technology, a probe is authorized to hybridise with its complementary DNA in the clone of cells. The cells are then inspected by autoradiography. The cells with mutated genes will not be identified on the photographic film because the probe was not complementary to the mutated genes.
• A probe is a single-stranded sequence of DNA or RNA utilized to search for its complementary sequence in a sample genome. The probe is positioned in contact with the sample under situations that authorize the probe sequence to hybridize with its corresponding sequence.

Hence, option B Recombinant DNA technology is the correct answer.