IN4: Which of the following statements about column
chromatography is correct?
2433283283
a) Resolution increases as the length of the
column increases
b) Mobile phase is a porous solid material with
appropriate chemical properties held in the column
c) Stationary phase is a buffered solution that
percolates through mobile phase
d) Large proteins emerge from the column sooner
than small ones
Answers
Answered by
1
Answer:
b is the answer write it
Answered by
0
Answer:
The resolution increase with increase the length of the column in column chromatography.
Therefore, the option (a) is correct.
Explanation:
- The principle of the column chromatography, the individual components move with different rates. The components have lesser affinity towards stationary phase will move faster than those have greater affinity towards stationary phase. The components move fast will be eluted first and the components move slow will be removed last.
- Mobile phase is made of solvents. It helps in the separation of components in the column.
- Stationary phase is porous solid material, it allows the mobile phase to flow freely have some absorption properties.
- Longer the length of the column more will be the resolution in column chromatography.
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