Question

Indicate best measurement way to determine the location of protein in the purification scheme

Answer 1

Explanation:

As most have mentioned, it depends on what you're purifying the protein from (recombinant from bacteria, from cell culture, etc.), whether you can add a tag for affinity purification, and how clean you need the protein. If you only need an enrichment and can tolerate some non-specific background proteins, 6xHis tags and Nickel resin work well and are cost effective. Although more expensive, FLAG tags or biotin acceptor peptides+strepavidin are good one-step methods to get a fairly clean prep. But I agree that more information about the protein, the starting material, and you're desired usage of the protein is required.