Investigatory project on study of pollen structure and calculation of pollen viability
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Real Lab Procedure
Prepare the pollen germination medium by dissolving 10 grams sucrose, 10 milligrams boric acid, 30 milligrams magnesium sulphate and 20 milligrams potassium nitrate in 100ml of distilled water.Using a glass rod, stir the solution to mix it well.Using a dropper, take some nutrient solution and put two drops on a clean glass slide.Take a mature flower and dust a few pollen grains from its stamen on to the drop on the slide.After 5 minutes, place the glass slide on the stage of the compound microscope.Observe the slide through the microscope regularly for about half an hour.
Prepare the pollen germination medium by dissolving 10 grams sucrose, 10 milligrams boric acid, 30 milligrams magnesium sulphate and 20 milligrams potassium nitrate in 100ml of distilled water.Using a glass rod, stir the solution to mix it well.Using a dropper, take some nutrient solution and put two drops on a clean glass slide.Take a mature flower and dust a few pollen grains from its stamen on to the drop on the slide.After 5 minutes, place the glass slide on the stage of the compound microscope.Observe the slide through the microscope regularly for about half an hour.
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