Question

ion exchange
chromatography with steps and diagram​

Answer 1

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Answer:

• An impure protein sample is loaded into the ion exchange chromatography column at a particular pH.
• Charged proteins will bind to the oppositely charged functional groups in the resin
• A salt gradient is used to elute separated proteins. At low salt concentrations, proteins having few charged groups are eluted and at higher salt concentrations, proteins with several charged groups are eluted.
• Unwanted proteins and impurities are removed by washing the column.

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Answer 2

Answer:

Explanation:

An impure protein sample is loaded into the ion exchange chromatography column at a particular pH.

Charged proteins will bind to the oppositely charged functional groups in the resin

A salt gradient is used to elute separated proteins. At low salt concentrations, proteins having few charged groups are eluted and at higher salt concentrations, proteins with several charged groups are eluted.

Unwanted proteins and impurities are removed by washing the column