Question

Is it like first the DNA from bacteria is removed then it is ligated with gene of interest, again the DNA is introduced in the same bacteria? And if not then how did the host accepts r-DNA if the r-DNA does not have a single portion of the host DNA?

Answer 1

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~* THE ANSWER OF YOUR QUESTION IS..*~

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YEP! YOU ARE RIGHT. ..

BUT THE PLASMID / BACTERIA. .WHICH IS SELECTED AS VECTOR. ..MUST HAVE
HIGH COPY NUMBER

ORIGIN OF REPLICATION

RESTRICTION SITES SUCH TETRACYCLIN , AMPICILLIN..ETC

SO THAT THEY CAN MULTIPLY IN THE HOST CELL EASILY AND PRODUCE HIGH COPY NUMBER.

FOR EXAMPLE:-

a bacteria named agrobacterium tumificians cause crown ball disease in plant ...
this bacteria were isolated and desired genes were introduced in it..inplace of disease causing gene...

HOPE IT WILL HELP YOU