Question

Is it possible to test a substance for the presence of starch without conducting iodine test? If yes, what are the different tests/ways?

Answer 1

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Answer 2

Answer:

Yes, there are alternative methods to test for the presence of starch in a substance without using iodine. Here are a few methods:

Saliva Test: Chew a small amount of the substance and hold it in your mouth for a few minutes. Enzymes in saliva break down starch into simpler sugars. Spit out the substance and add a few drops of water to it. Then, add a few drops of iodine solution. If starch is present, the mixture will turn blue-black.

Potassium Permanganate Test: Mix a small amount of the substance with a few drops of dilute sulfuric acid, followed by a few drops of potassium permanganate solution. Heat the mixture gently. If starch is present, the purple color of the potassium permanganate solution will disappear.

Copper Sulfate Test: Mix a small amount of the substance with a few drops of water and a few drops of copper sulfate solution. Heat the mixture gently. If starch is present, a reddish-brown precipitate of copper starch will form.

Enzyme Test: Mix a small amount of the substance with a few drops of water and a few drops of amylase enzyme solution. Incubate the mixture at 37°C for a few minutes. Then, add a few drops of iodine solution. If starch is present, the mixture will turn blue-black. The enzyme breaks down starch into simpler sugars that can no longer react with iodine.

These methods are not as sensitive or specific as the iodine test, but they can provide a rough estimate of the presence of starch in a substance.