Is it safe to mix bromothyl blue & mono sodium glutamte?
Answers
Explanation:
Bromothymol blue is used to determine the pH of a solution. In this lesson we will learn how to prepare it for use as well as safety precautions to take while preparing it.
Bromothymol Blue Background
There are several methods to determine the pH of a sample. Color changing methods are nice, because they are quick and the results can easily be seen. They are also helpful because we can watch the color change as the pH changes (thus tracking pH changes visually). Bromothymol blue is a compound that tests the pH using color changes. It is made in a basic solution, so it starts out as blue, thus the name 'blue'. It changes to yellow in acidic conditions.
When bromothymol blue is basic it has a blue color, as the solution becomes more acidic it changes to a yellow color
Color change
Bromothymol blue can also be used for measuring respiration and photosynthesis. As carbon dioxide increases, the solution turns yellow.
Bromothymol Blue Preparation
Bromothymol blue is a large compound with three benzene rings with two bromines (where the 'bromo' in the name comes from), a sulfur attached to three oxygens (a 'thym' group), and an alcohol (the -ol suffix). It is a powder, which makes it difficult to mix in with the sample to test for the pH. So, in order to prepare bromothymol blue, it needs to be made into an aqueous solution.
The structure of bromothymol blue
Structure
Reagents
0.1 g Bromothymol blue powder
16mL 0.01 N sodium hydroxide
Distilled Water
Steps
Dissolved bromothymol powder in sodium hydroxide
Diluted with water up to 250 mL
Calculations
The above measurements will make a 0.04% bromothymol blue solution. Since we fill up to 250 mL of water the entire solution ends up making up 250 mL, and we know that there is 0.1 g of bromothymol blue in the solution. 0.1/250 = 0.0004 or 0.04%. The 0.04% is a common concentration used, but sometimes 0.01% is also used, in order to do this we would simply need to increase the amount of water we add. In order to determine the new 'fill-to' amount we can calculated the unknown:
0.1/x = 0.0001
0.1 = 0.0001x
0.1/0.0001 = x
1000 = x
So we will need to fill up to 1000 mL (1 L) of distilled water.
Answer: