Question

Is RdRp from Influenza A inserted into a mammalian host's nucleus or derived from inserted viral RNA?,​

Answer 1

Answer:

Upon penetration of the influenza virus nucleocapsid into the host cell cytoplasm, the viral RNA and associated proteins are transported to the nucleus, where viral transcription and replication occur.

Explanation:

1. Yes, RdRp is inserted into the nucleus alongside the vRNA.

2. RdRp serves as its own factory but not completely unassociated ; cap snaching from host pre mRNA is example

3. Viral capsule remains in the nucleus . Only viral genome enters and replicate inside the nucleus.

Answer 2

Answer:

This paper describes in part what is going on with Influenza A:

Article Influenza A: Understanding the Viral Life Cycle

and says, "The influenza viral genome is made up of negative sense strands of RNA. In order for the genome to be transcribed, it first must be converted into a positive sense RNA to serve as a template for the production of viral RNAs.

Replication of the genome does not require a primer; instead, the viral RNA dependent RNA polymerase (RdRp) initiates RNA synthesis internally on viral RNA. This is possible, as the extreme 5’ and 3’ ends of the genome exhibit partial inverse complementarity and, hence, are able to base pair with one another to form various corkscrew configurations. It appears that a great number of di-nucleotide base pairs form, although the full mechanism of viral genome replication is still yet to be understood"

Does this mean that the RdRp is inserted in the nucleus along with the viral RNA, or derived from it inside the nucleus? Does the RdRp serve as its own 'factory', separate from the host's own transcription mechanism? Or does the RdRp replicate the viral RNA inside the viral capsule which is still out in the cytoplasm, and only viral RNA gets inserted into the nucleus?