Question

is syringaldazine soluble in water?

Answer 1

The assay mixture contained 0.2 mM syringaldazine (dissolved in 60% ethanol) and 40 mM citrate-buffer, pH 5.6. Oxidation of syringaldazine was followed by an absorbance increase at 525 nm (ϵ525 = 65 000 dm3 M−1 cm−1) at a temperature of 25°C" Ignoring what is stated in the link (which confirms the idea) I doubt that water would interfere, since the molecule is stable in water, and since the enzyme works in a environment in which water is present