Isolation and purification of chromosomal and plasmid dna
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Purification of the plasmid DNA. It involves growth of the bacterial cells in a media containing essential nutrients. ... Addition of acetate-containing neutralization buffer results in the precipitation of large and less supercoiled chromosomal DNA and proteins leaving the small bacterial DNA plasmids in solution.
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Principle. Purification of plasmid DNA from bacterial DNA using is based on the differential denaturation of chromosomal and plasmid DNA using alkaline lysis in order to separate the two. ... Bacteria are lysed with a lysis buffer solution containing sodium dodecyl sulfate (SDS) and sodium hydroxide.
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