Question

Isolation of bacteria from rhizospheric soil of mentha arvensis

Answer 1

The aim of this study was to the isolate the endophytic bacteria, optimize its isolation procedure and preliminary characterize the isolated endophytes. Methods: Ethanol, sodium hypochlorite, and mercuric chloride at various concentrations and duration were employed to optimize the surface sterilization for the isolation of endophytes from Catharanthus roseus, Ocimum sanctum, Mentha arvensis, and Stevia rebaudiana. Microscopic and biochemical characterization of isolates were carried out. Results: A total of 35 endophytic bacteria have been isolated from four medicinal plants. Combination of 2% sodium hypochlorite, 70% ethanol, and 0.1% mercuric chloride was found effective for the surface sterilization of C. roseus and O. sanctum. In case of M. arvensis and S. rebaudiana, 70% ethanol and 2% sodium hypochlorite was found suitable for the surface sterilization. In preliminary screening 18 isolates were found Gram-positive cocci, 11 were Gram-negative bacilli, 6 were found to be Gram-positive bacilli, 18 isolates showed positive results for endospore staining, 24 gave positive results for catalase test, 13 gave positive results for oxidase test, and 15 isolates were found to be motile.


Conclusion: Ethanol, sodium hypochlorite, and mercuric chloride were found effective decontaminating agents in optimum condition. In the preliminary screening diverse colony, different shapes, color, margins, and textures were observed. 2015, Asian Journal of Pharmaceutical and Clinical Research. All rights reserved.


please follow me guys