Isolation of lipids from exosomes mycobacterium tuberculosia
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Thanks for the question!
It is definitely a very interesting question to solve and do some brainstorming.
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Mycobacterium tuberculosis is a bacterial rod-shaped gram positive bacteria and a intracellular pathogenwhere the pathogenic substance is primarily destroying the infected tissue and releases highly toxic chemical called tuberculin or a test called Mantoux reaction which has identified tuberculin specific toxins in the lung and some other tissues. Isolation of lipids from exosomes, first it will require the exosomes to be isolated, we have one popular medium for extracting exosomes, that is, from the species Mus musculus this organism is named as "RAW 264.7" and releases lysozyme as a cellular product which has the property of adherence, it is then isolated and extraction is done from Mycobacterium bovis with treated mice from PBS. The exosomes are then purified from the serum obtained by the mouse by creating buffer solutions from a media, serum and other required sources to obtain high quality exosomes. Mycobacterial lipids are used for further isolation of lipid from exosomes. Mycobacterium bovis are rather extricated from the cell wall lipid from a BCG vaccine when inserted with a matrix containing gel substance. The lipids which are extracted and isolated for tissue culture are very small upto 30-90 nanometer with membrane vesicles which are performed through fusion of MVBs or multivesicular bodies with plasma membranes and the total release of intraluminal cells and vesicles as exosomes, the lipid is isolated from mixtures of chloroform and methanol solutions.
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Hope it helps and solves your query!!
It is definitely a very interesting question to solve and do some brainstorming.
**************************************************
Mycobacterium tuberculosis is a bacterial rod-shaped gram positive bacteria and a intracellular pathogenwhere the pathogenic substance is primarily destroying the infected tissue and releases highly toxic chemical called tuberculin or a test called Mantoux reaction which has identified tuberculin specific toxins in the lung and some other tissues. Isolation of lipids from exosomes, first it will require the exosomes to be isolated, we have one popular medium for extracting exosomes, that is, from the species Mus musculus this organism is named as "RAW 264.7" and releases lysozyme as a cellular product which has the property of adherence, it is then isolated and extraction is done from Mycobacterium bovis with treated mice from PBS. The exosomes are then purified from the serum obtained by the mouse by creating buffer solutions from a media, serum and other required sources to obtain high quality exosomes. Mycobacterial lipids are used for further isolation of lipid from exosomes. Mycobacterium bovis are rather extricated from the cell wall lipid from a BCG vaccine when inserted with a matrix containing gel substance. The lipids which are extracted and isolated for tissue culture are very small upto 30-90 nanometer with membrane vesicles which are performed through fusion of MVBs or multivesicular bodies with plasma membranes and the total release of intraluminal cells and vesicles as exosomes, the lipid is isolated from mixtures of chloroform and methanol solutions.
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Hope it helps and solves your query!!
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