It is because of the requirement of the origin of replication that a pieceof DNA if needed to be propagated during recombinant DNA procedures, requires a vector. The vectors provide the origin of replication. PLEASE explain this in easy words and with detailed explanation..
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Recombinant DNA and genetic techniques
Recombinant DNA (or rDNA) is made by combining DNA from two or more sources. ...
For in vivo cloning a fragment of DNA, containing a single gene or a number of genes, is inserted into a vector that can be amplified within another host cell. ...
A gene library is a large collection of cloned DNA sequences from a single genome.
I guess this would be a correct answer
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