Question

Keller-kiliani test for cardiac glycosides

Answer 1

Keller-Kiliani Test

Explanation:

• Keller's reagent is the find different kinds of Alkaloids via reactions, which produced the products with the colors. The Cohn defined the theory, its use to identify, the principal components of digitalis.so the reaction is known as Keller–Kiliani reaction.
• The glycolysis will be confirmed when the color test A is more intense than test B.  
• The sugar (glycoside) and the non-sugar (aglycon) moieties are found in The cardiac glycosides.

Answer 2

Keller-killiani tests for cardiac glycosides are:

• Glycoside is a molecule in which sugar is bound to another active group by a glycoside bond.
• Glycosides play many important roles in living things.

Keller-Kiliani Examination of Deoxysugars:

• Deoxysugar (found in cardiac glycosides) is soluble in acetic acid containing a trace of ferric chloride and transferred over concentrated sulphuric acid.

• When the liquid mixes it produces a reddish-brown colour that gradually becomes blue.

Killer killiani test:

• Glycoside dissolves in a mixture of 1% ferric sulphate solution in (5%) of glacial acetic acid.
• Add one or two drops of concentrated sulphuric acid.
• The blue colour grows due to the presence of deoxy sugar.