Krishna is working on a protein, which is a potential DNA interactor. He sought advice from another researcher but the steps of process were NOT in sequence. How will you arrange the below given steps to help Krishna identify DNA stretches where his partially purified protein can interact?
i) Sequencing of fragmented DNA
ii) Incubation for protein binding
iii) Fragmentation of DNA
iv) Alignment of sequence of reference genome
v) Immunoprecipitation with antibody specific to the target protein
Answers
Answer:
v is right answer that is immunoprecipotation with antibody specific to the targer protein
Answer:
The correct order is ii) - iii) – iv) – i) – v)
Explanation:
The correct order of steps will be as follows:
ii) Incubation for protein binding – After the protein was introduced, the DNA was kept in incubation to allow for binding of the protein.
iii) Fragmentation of DNA – After the binding of the protein, the DNA was fragment using suitable enzymes.
iv) Alignment of sequence of reference genome – The reference genome is aligned as per the sequence to aid in the alignment of the original genome.
i) Sequencing of fragmented DNA – The fragmented DNA was then aligned in accordance with the sequence of the alignment of the reference genome.
v) Immuno-precipitation with antibody specific to the target protein – Immunoprecipitation of the DNA fragments with antibody specific to the target protein will help in identifying the DNA stretches.