Question

Lactic acid bactria and bifidobacterium reserachgate

Answer 1

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The isolation and identification of lactic acid bacteria (LAB) in the honey stomachs and honeycombs of Giant honeybee (Apis dorsata) from different areas in Malaysia were performed to prospect for beneficial bacteria or probiotics. Honey stomach samples of honeybee and honeycomb filled with honey were collected from Apis dorsata colonies in different bee trees from the rainforests of Pedu Lake in Kedah state and the agricultural region of Marang in Terengganu, Malaysia. The isolates were cultured anaerobically in different media of MRS agar, MRS broth and TPY broth at 37°C. Three hundred and fourteen isolates were obtained and identified based on biochemical tests, as LAB. In addition, 20 uncultured samples were identified as Bifidobacterium by using the nested polymerase chain reaction (PCR). The 16S rRNA genes from extracted DNA of bacterial colonies were amplified with PCR and nested PCR using universal primers of 27F and 1492R for LAB, and for Bifidobacterium two pairs of genus specific primers (Lm26 paired with Lm3 and Bif164-F paired with Bif662-R) were utilized. All bacterial 16S rRNA genes were sequenced and entrusted in GenBank and were given specific accession numbers. Phylogenetic analysis of the 16S rRNA sequences showed that the novel LAB and

Answer 2

Answer:

The isolation and identification of lactic acid bacteria (LAB) in the honey stomachs and honeycombs of Giant honeybee (Apis dorsata) from different areas in Malaysia were performed to prospect for beneficial bacteria or probiotics. Honey stomach samples of honeybee and honeycomb filled with honey were collected from Apis dorsata colonies in different bee trees from the rainforests of Pedu Lake in Kedah state and the agricultural region of Marang in Terengganu, Malaysia. The isolates were cultured anaerobically in different media of MRS agar, MRS broth and TPY broth at 37°C. Three hundred and fourteen isolates were obtained and identified based on biochemical tests, as LAB. In addition, 20 uncultured samples were identified as Bifidobacterium by using the nested polymerase chain reaction (PCR). The 16S rRNA genes from extracted DNA of bacterial colonies were amplified with PCR and nested PCR using universal primers of 27F and 1492R for LAB, and for Bifidobacterium two pairs of genus specific primers (Lm26 paired with Lm3 and Bif164-F paired with Bif662-R) were utilized. All bacterial 16S rRNA genes were sequenced and entrusted in GenBank and were given specific accession numbers. Phylogenetic analysis of the 16S rRNA sequences showed that the novel LAB and