Ligase enzyme is used for –
(a) denaturation of DNA
(b) splitting DNA into small bits
(c) joining bits of DNA
(d) digestion of lipids
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Ligase enzyme is used for joining bits of DNA.
* It has a major role in DNA replication as well as DNA repair as it helps in joining smaller DNA fragments.
* During DNA replication, it joins the Okazaki fragments in the lagging strand synthesis.
* It functions by the formation of a bond between deoxyribose group on one strand and the phosphate group on the other.
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