Linear dna closed circular and single stranded dna. which runs faster
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Plasmid DNA shows two distinct conformations: Supercoiled, where DNA is wound up into a compact structure; and Nickcircle, where one strand is a covalently continuous circle and the other has been cut; either in the form of monomers or
dimers. In the Supercoiled structure (or covalently closed circular structure), DNA is fully intact with both strands uncut with a built in twist, resulting in a twisted compact form where the strands may become more tightly or more loosely wound together accommodating one too many or one too few helical twists. The Nickcircle form is one of the most relaxed and unstressed states of a closed-circular DNA and shows the slowest electrophoretic mobility for uncut plasmid DNA. The Supercoiled DNA shows the highest electrophoretic mobility in an agarose gel and represents 95% of all plasmid DNA conformations (in Conde et al. "Modification of plasmid DNA topology by 'histone-mimetic' gold nanoparticles" )
Besides all this you can only know the exact size of the plasmid if you digest with a restriction enzyme that cuts only once your plasmid
dimers. In the Supercoiled structure (or covalently closed circular structure), DNA is fully intact with both strands uncut with a built in twist, resulting in a twisted compact form where the strands may become more tightly or more loosely wound together accommodating one too many or one too few helical twists. The Nickcircle form is one of the most relaxed and unstressed states of a closed-circular DNA and shows the slowest electrophoretic mobility for uncut plasmid DNA. The Supercoiled DNA shows the highest electrophoretic mobility in an agarose gel and represents 95% of all plasmid DNA conformations (in Conde et al. "Modification of plasmid DNA topology by 'histone-mimetic' gold nanoparticles" )
Besides all this you can only know the exact size of the plasmid if you digest with a restriction enzyme that cuts only once your plasmid
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