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list 6 advantages of plant tissue culture... please don't spam..... ​

Answers

Answered by dineshsahu8024
18

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Advantages of Tissue Culture

1. The plantlets are obtained in a very short time with a small amount of plant tissue.

2. The new plants produced are disease-free.

3. The plants can be grown throughout the year, irrespective of the season.

4.A large space is not required to grow plants by tissue culture technique.

5. It may be vulnerable to many environmental factors since they have same genetic material.

6. It is labor intensive and expensive process.

Hope it's help you

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Answered by renjithsonukrishna
3

Answer:

What is Tissue Culture?

Tissue culture is a technique in which fragments of plants are cultured and grown in a laboratory. Many times the organs are also used for tissue culture. The media used for the growth of the culture is broth and agar.

This technique is also known as micropropagation. It has proved beneficial for the production of disease-free plants and increase plant yield in developing countries. It only requires a sterile workplace, greenhouse, trained manpower, and a nursery.

Oil palm, banana, eggplant, pineapple, rubber tree, tomato, sweet potato have been produced by tissue culture in the developing countries.

Types of Tissue Culture

Following are the different types of tissue culture techniques:

Seed Culture

In this culture, the explants are obtained from an in-vitro derived plant and introduced into a laboratory where they proliferate. The explant should be sterilized to prevent it from tissue damage.

Embryo Culture

This involves the in-vitro development of an embryo. For this, an embryo is isolated from a living organism. Both, a mature or an immature embryo can be used in the process. Mature embryos can be obtained from ripe seeds. The immature embryos are obtained from the seeds that failed to germinate. The ovule, seed or fruit is already sterilized, therefore, it does not need to be sterilized again.

Callus Culture

A callus is an unorganized, dividing mass of cells. When the explants are cultured in a proper medium, the callus is obtained. The growth of callus is followed by organ differentiation. The culture is grown on a gel-like medium composed of agar and specific nutrients required for the growth of the cells.

Organ Culture

In this, any organ of the plant such as shoot, leaf, can be used as an explant. A number of methods can be used for the organ culture, such as plasma clot method, raft method, grid method, and agar gel method. This method is used to preserve the structure and functions of an organism.

Protoplast Culture

It is a cell without a cell wall. A protoplast can be cultured using the hanging-drop method, or micro-culture chambers. In protoplast culture, a number of phases can be observed: development of cell wall, cell division, regeneration of a whole plant.

Other Types

Pollen Culture

Anther Culture

Single Cell Culture

Suspension Culture

Somatic Embryogenesis

Steps of Tissue Culture

The steps of tissue culture are given below:

Initiation Phase

At this stage, the tissue is initiated into the culture. The tissue of interest is obtained, introduced and sterilized to prevent the process from any contamination.

Multiplication Phase

In this stage, the sterilized explant is introduced into the medium composed of growth regulators and appropriate nutrients. They are responsible for the multiplication of cells. This undifferentiated mass of cells is known as a callus.

Root Formation

The roots start forming. Plant growth hormones are added to initiate the root formation. Consequently, we obtain a complete plantlets.

Shoot Formation

The plant growth hormones for the formation of shoot are added and the growth is observed for a week.

Acclimatization

When the plant starts developing, it is transferred to the greenhouse to develop under controlled environmental conditions. It is finally transferred to the nurseries to grow under natural environmental conditions.

Advantages of Tissue Culture

Following are the various advantages of tissue culture technique:

The plantlets are obtained in a very short time with a small amount of plant tissue.

The new plants produced are disease-free.

The plants can be grown throughout the year, irrespective of the season.

A large space is not required to grow plants by tissue culture technique.

The production of new varieties in the market place speeds up.

This technique is being used for the production of ornamental plants such as dahlia, chrysanthemum, orchids, etc.

Step-by-step explanation:

i hope u understand

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