list out the apparatus ( materials and chemicals ) you used in the observations of the root tip experiment? explain briefly?
Answers
Explanation:
Aim Of The Experiment
To study and demonstrate mitosis by preparing the mount of an onion root tip cells.
Theory Of The Experiment
For entities to mature, grow, maintain tissues, repair and synthesize new cells, cell division is required. Cell division is of two types:
Mitosis
Meiosis
Materials Required
Compound microscope
Acetocarmine stain
Water
Burner
N/10 Hydrochloric acid
Filter paper
Coverslip
Aceto alcohol (Glacial acetic acid and Ethanol in the ratio 1:3)
Glass Slide
Onion root peel
Forceps
Blade
Dropper
Needle
Vial
Explanation:
Aim Of The Experiment
To study and demonstrate mitosis by preparing the mount of an onion root tip cells.
Theory Of The Experiment
For entities to mature, grow, maintain tissues, repair and synthesize new cells, cell division is required. Cell division is of two types:
Mitosis
Meiosis
materials required:
Compound microscope
Acetocarmine stain
Water
Burner
N/10 Hydrochloric acid
Filter paper
Coverslip
Aceto alcohol (Glacial acetic acid and Ethanol in the ratio 1:3)
Glass Slide
Onion root peel
Forceps
Blade
Watch glass
Dropper
Needle
Vial
procedure of the experiment:
Place an onion on a tile
With the help of a sharp blade, carefully snip the dry roots of the onion
Place the bulbs in a beaker containing water to grow the root tips
It may take around 4 to 6 days for the new roots to grow and appear
Trim around 3 cm of the newly grown roots and place them in a watch glass
With the help of forceps, shift it to a vial holding freshly prepared aceto-alcohol i.e., a mixture of glacial acetic acid and ethanol in the ratio 1:3.
Allow the root tips to remain the vial for one complete day
With the help of forceps, pick one root and set in on a new glass slide
With the help of a dropper, allow one drop of N/10 HCl to come in contact with the tip of the root. Additionally, add around 2 to 3 drops of the acetocarmine stain.
Heat is lightly on the burner in such a way that the stain does not dry up.
Excessive stain can be carefully treated using filter paper
The more stained part of the root tip can be trimmed with the help of a blade.
Discard the lesser stained part while retaining the more stained section.
Add a droplet of water to it
With the help of a needle, a coverslip can be mounted on it
Gently tap the coverslip with an unsharpened end of a needle in order for the meristematic tissue of the root tip present under the coverslip to be squashed properly and to be straightened out as a fine cell layer
The onion root tip cells’ slide is now prepared and ready to be examined for different stages of mitosis
Observe and study mitosis by placing the slide under the compound microscope. Focus as desired to obtain a distinct and clear image.