Question

List out the apparatus (meterials and chemicals) you used in the observions of root tip experiment? Explane briefly​

Answer 1

Answer:Aim Of The Experiment

To study and demonstrate mitosis by preparing the mount of an onion root tip cells.

Theory Of The Experiment

For entities to mature, grow, maintain tissues, repair and synthesize new cells, cell division is required. Cell division is of two types:

Mitosis

Meiosis

materials required:

Compound microscope

Acetocarmine stain

Water

Burner

N/10 Hydrochloric acid

Filter paper

Coverslip

Aceto alcohol (Glacial acetic acid and Ethanol in the ratio 1:3)

Glass Slide

Onion root peel

Forceps

Blade

Watch glass

Dropper

Needle

Vial

procedure of the experiment:

Place an onion on a tile

With the help of a sharp blade, carefully snip the dry roots of the onion

Place the bulbs in a beaker containing water to grow the root tips

It may take around 4 to 6 days for the new roots to grow and appear

Trim around 3 cm of the newly grown roots and place them in a watch glass

With the help of forceps, shift it to a vial holding freshly prepared aceto-alcohol i.e., a mixture of glacial acetic acid and ethanol in the ratio 1:3.

Allow the root tips to remain the vial for one complete day

With the help of forceps, pick one root and set in on a new glass slide

With the help of a dropper, allow one drop of N/10 HCl to come in contact with the tip of the root. Additionally, add around 2 to 3 drops of the acetocarmine stain.

Heat is lightly on the burner in such a way that the stain does not dry up.

Excessive stain can be carefully treated using filter paper

The more stained part of the root tip can be trimmed with the help of a blade.

Discard the lesser stained part while retaining the more stained section.

Add a droplet of water to it

With the help of a needle, a coverslip can be mounted on it

Gently tap the coverslip with an unsharpened end of a needle in order for the meristematic tissue of the root tip present under the coverslip to be squashed properly and to be straightened out as a fine cell layer

The onion root tip cells’ slide is now prepared and ready to be examined for different stages of mitosis

Observe and study mitosis by placing the slide under the compound microscope. Focus as desired to obtain a distinct and clear image.

Explanation: