Question

List out the factors affecting melting temperature of dna

Answer 1

(The other is RNA. In humans DNA is the genetic material; RNA is transcribed from it. In some other organisms, RNA is the genetic material and, in reverse fashion, theDNA is transcribed from it.) DNA is a double-stranded molecule held together by weak hydrogen bonds between base pairs of nucleotides

Answer 2

The melting point of DNA is connected to the GC content of it.

Since the GC forms 3 hydrogen bonds while AT forms two hydrogen bonds, the more GC you have in your DNA high the melting point will be.

For example if the GC content is about 50- per cent then melting point is approximately around 50c.