List out the steps involved in Ex - vivo gene therapy
Answers
Answer:
Steps involved in the “same day” ex vivo gene therapy. (a) Harvest of bone marrow from the rat femur; (b) Ficoll separation and preparation of rat “same day” bone marrow cells (SD-RBMCs) for viral transduction (time required 0.5 hour); (c) Short-duration viral transduction of SD-RBMCs (time required 1 hour); (d) Post-transduction preparation of SD-RBMCs (time required 1 hour); (e) Placement of transduced SD-RBMCs on a collagen-ceramic matrix and implantation into the femoral defect; (f) Healed femoral defect at 8 weeks after cell implantation. SD-RBMCs, “same day” rat bone marrow cells.
Steps involved in the “same day” ex vivo gene therapy. (a) Harvest of bone marrow from the rat femur; (b) Ficoll separation and preparation of rat “same day” bone marrow cells (SD-RBMCs) for viral transduction (time required 0.5 hour); (c) Short-duration viral transduction of SD-RBMCs (time required 1 hour); (d) Post-transduction preparation of SD-RBMCs (time required 1 hour); (e) Placement of transduced SD-RBMCs on a collagen-ceramic matrix and implantation into the femoral defect; (f) Healed femoral defect at 8 weeks after cell implantation. SD-RBMCs, “same day” rat bone marrow cells.
Source publication
Figure 1: Steps involved in the “same day” ex vivo gene therapy. (a)...
table 1 radiographic results
Figure 2: Structure of lentiviral two-step transcription amplification...
table 2 torsional biomechanical testing
Figure 3: Plain radiographs. (a) Representative radiographic images of...
“Same Day” Ex-vivo Regional Gene Therapy: A Novel Strategy to Enhance Bone Repair