Question

list the step of preparation of temporary mount of a leaf peel to observe stomata

Answer 1

Stomata are small openings found widely scattered on the epidermis of leaves and young stems. They are mostly found on the lower surface of a dicot leaf and on both the surfaces of a monocot leaf. Stomata regulate the exchange of gases and water vapour between the atmosphere and leaves.

Procedure:

1. Remove a healthy leaf from the potted plant.
2. Remove a part of the peel from the lower surface of the leaf. You can do this by folding the leaf over and gently pulling the peel apart using forceps. Keeps the peel in a watch glass containing water.

Answer 2

peel off the leaf  

place the peel on the slide

add drop of water

then add glycerine and safranin

cover with cover slip

then see under microscope