Question

List the steps for preparing a slide for an onion peel

Answer 1

1 Take a piece of onion and bend it to remove the transparent membranous structure called onion epidermal peel. 2 With help of forcep remove the peel from its inner side.

3 Place the peel in water in a watch glass.

4 Add a few drops of stain safranin, to the watch glass containing the peel for staining.

5 Now, wash the leaf peel with water and transfer it on to a clean slide with the help of brush.

6 Remove extra water from the slide surrounding the peel with the help of blotting paper.

7 To this slide, add a drop of glycerine over the peel and place the coverslip in a manner to avoid entry of air bubbles.

Soak away the extra glycerine with blotting paper.

8 Examine slide under the microscope

hope this will help u