Question

list the steps of preparation of temporary mount of a leaf peel to observe stomata??

Answer 1

1. Remove a healthy leaf from the potted plant.

2. Remove a part of the peel from the lower surface of the leaf. You can do this by folding the leaf over and gently pulling the peel apart using forceps. Keeps the peel in a watch glass containing water.

3. Put a few drops of safranin stain in a watch glass.

4. After 2-3 minutes take out the peel and place it on a clean glass slide.

5. Put a drop of glycerin over the peel and place a clean coverslip gently over it with the help of a needle.

6. Remove the excess stain and glycerin with the help of blotting paper.

7. Observe the slide under the low-power and high-power magnifications of the compound microscope.

Answer 2

1 - Remove a healthy leaf from a potted plant .
2-remove a part of peel from the lower surface of the leaf . keep the peel in a watch glass . 
3- put a few drops of safranin stain in a watch glass 
4- after 2 -3 mins take out the peel and place it on a clean glass slide . 
5- put a drop of glycerin on the peel and place a clean cover slip gently over it with the help of a needle . 
6- remove the excess stain an glycerin with the help of blotting paper .
7- observe the slide under the low power and high power magnifications of the compound microscope 


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