Question

List the steps to be followed in preparing a temporary mount of onion cells.

Answer 1

1. Take an onion and remove its outermost peel.

2. Now cut a small part from an inner scale leaf with the help of a blade.

3. Separate a thin, transparent peel from the convex surface of the scale leaf with the help of forceps.

4. Keep this peel in a watch glass containing water

5. Add two drops of safranin stain in the watch glass to stain the peel.

6. Take a clean slide and put a drop of glycerine in the centre of the slide.

7. With the help of a brush and needle transfer the peel on the slide. Glycerine prevents the peel from drying up.

8. Carefully cover it with a coverslip and avoid any air bubble from entering interring the coverslip.

9. Remove any excessive glycerine with a filter paper.

10. Observe the prepared mount of the peel under the low and high magnification of a compound microscope.

Answer 2

Hey friend,

Here's your answer,


TO CREATE A TEMPORARY MOUNT OF ONION PEEL, FOLLOW THE GIVEN STEPS :-


✴ Take an onion scale from an onion bulb.


✴ Break the concave side of onion scale in two till a thin translucent membrane is separated.


✴ Tear this thin onion peel or epidermis into pieces.


✴ Keep a piece of peel on the glass slide and put a drop of methylene blue stain on it.


✴ Put a drop of glycerine on stained peel and place a coverslip on it.


✴ Clean the slide from the edges and observe the mount first under low power of microscope and then under high power of microscope.



Hope this helps!!!