Lymphocytes separated from whole blood by lysing the red blood cells (rbcs) using a hypotonic buffer (ammonium bicarbonate and ammonium chloride; himedia) with minimal lysing effect on lymphocytes.
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Several studies have suggested that bone marrow stromal steam cells (BMSC) exist in a quiescent state (G0) within the in vivo niche; however, an explicit analysis of the biology.
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