magnesium phosphate transfer of electrons
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Phosphate transfer enzymes generally contain a Mg2+ ion bound in the active site in a position where it can interact with non-bridging phosphate oxygens on the substrate. The magnesium ion pulls electron density away from the phosphorus atom, making it more electrophilic.
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The magnesium ion pulls electron density away from the phosphorus atom, making it more electrophilic. Without this metal ion interaction, a phosphate is actually a poor electrophile, as the negatively-charged oxygens shield the phosphorus center from attack by a nucleophile
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