make a slide of a stomata and observe it.
Answers
Answer:
In the watch glass, stain the sample by adding some drops of safranin through a dropper. Add a drop of glycerin on the peel. Put a clear coverslip over it gently using a needle. Examine the slide first under a low-power and then under a high-power magnification of a compound microscope.
Explanation:
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Stomata are microscopic apertures or pores that can be seen on the epidermis of leaves and on young stems.
Explanation: Aim: To make a temporary mount of a leaf peel so that the stomata of a leaf can be seen.
Materials: A leaf, glycerine, microscope, glass slides, watch glass, brush, dropper, blotting paper, safranin.
Method: Pluck a healthy leaf from the plant. To remove a peeled section from the lower surface of the leaf, fold the leaf in half and gently pull the peel away. To complete this stage, use forceps. Allow the peel to soak for a while in a watch glass filled with water.
Apply a few drops of safranin to the sample in the watch glass with a dropper.
After 2-3 minutes, remove the peel. Place it on a transparent glass slide.
Using a drop of glycerin, apply it on the peel. Using a needle, lightly cover it with a clear coverslip.
Blotting paper can be used to remove excess glycerin and discoloration.
Examine the slide with a compound microscope, first at low magnification and then at high magnification.
Observation: On the lower surfaces of the leaf, epidermal cells with stomata can be detected.
