Question

Make a temporary stained squash preparation of onion root tip. Identify, sketch and
label any one stage of mitosis. Show your preparation to the examiner.​

Answer 1

Explanation:

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Answer 2

our aim is to prepare a temporary mount slide of onion root tips for the study of mitosis.

Explanation:

Requirement

acetocarmine stain,burner, water,N/10 hydrochloric acid,cover slip,aceto alchol,filter paper,microscope,onion root tips,

Procedure

Cut off 2–3 cm of freshly grown roots and let them drop into a watch glass.

then Using a forceps, transfer them freshly prepared fixative of aceto-alcohol ().

rest the root tips for  for 24 hours.

Using a dropper, place one drop of N/10 HCl on the root tip followed by 2–3 drops of acetocarmine stain.

Warm it slightly on burner. Care should be taken that the stain is not dried up.

blot the excess stain using filter paper.

Using a blade, cut the comparatively more stained tip portion of the root, retain it on the slide and discard the remaining portion.

After that, put one drop of water on the root tip.

Now, slowly tap the cover slip using the blunt end of a needle so that the meristematic tissue of the root tip below the cover slip is properly squashed and spread as a thin layer of cells.

This preparation of onion root tip cells is now ready for the study of mitosis.

Place the slide under the compound microscope and observe the different stages of mitosis.

Various stages of mitosis are prophase, metaphase, anaphase and telophase.