mass spectrometer full instrumentation
Answers
Explanation:
Mass spectrometry uses an instrument called a mass spectrometer. The main components of a mass spectrometer are: Inlet system (LC, GC, Direct probe etc...) Ion source (EI, CI, ESI, APCI, MALDI, etc...)
Answer:
What is Mass Spectrometry?
Mass spectrometry is an analytical technique that involves the study in the gas phase of ionized molecules with the aim of one or more of the following:
Molecular weight determination
Structural characterization
Gas phase reactivity study
Qualitative and quantitative analysis of components in a mixture.
Mass spectrometry consists basically of weighing ions in the gas phase. The instrument used could be considered as a sophisticated balance which determines with high precision the masses of individual atoms and molecules. Depending on the samples chemical and physical properties, different ionization techniques can be used. One of the main factor in choosing which ionization technique to be used is thermolability. For samples that are not themolabile and relatively volatile, ionization such as Electron Impact and/or Chemical Ionization can be effectively used. For samples that are thermolabile such as peptides, proteins and other samples of biological interest, soft ionization techniques are to be considered. Among the most used soft ionization techniques are Electrospray (ESI) and Matrix Assisted Laser Desorption (MALDI). The name given to a particular mass spec technique is usually pointing to the ionization method being used.
Atomic and molecular masses are assigned relative to the mass of the carbon isotope, 12C, whose atomic weight is defined as exactly 12. The actual mass of 12C is 12 daltons, with one dalton is equal to 1.661 10-24 g. The mass of a molecule or an ion can be presented in daltons (Da) or kilodaltons (kDa).