Question

Melting temperature in nucleotide is given by formula

Answer 1

Answer:

Explanation:

Tm = 4(G+C) +2(A+T), by calculating all the number of G, C, A, T in your desired sequence.. calculate for both the primers and then take the average, it will give you melting tempt. Tm and to get annealing temperature for PCR it is around 5 degree less than Tm.